Education:

 B.S., 1988
University of Minnesota, St, Paul, MN
College of Biological Sciences
Genetics and Cell Biology
 Ph.D., 1994
Iowa State University, Ames, IA
Molecular, Cellular, and Developmental Biology
Department of Botany
Advisor: Dr. James Colbert
Postdoctorate
1995-1999
NIH Postdoctoral Fellow
Boyce Thompson Institute for Plant Research
Cornell University, Ithaca, NY
Advisors: Dr. David Stern and Dr. Karen Kindle


Publications:

Higgs, D.C. (2009) The Chloroplast Genome, Chapter 24. In, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes, 2nd Edition Volume 2, Ed. David B. Stern. Academic Press.

Pham, D.Q.-D., Higgs, D.C., Statham, A., Schleiter, M.K. (2008) Implementation and assessment of a molecular biology and bioinformatics undergraduate degree program. Biochem. Mol. Biol. Educ., 36: 106-115.

Rymarquis, L.A., Higgs, D.C., Stern, D.B. (2006) Nuclear suppressors define three factors that participate in both 5' and 3' end processing of mRNAs in Chlamydomonas chloroplasts. The Plant Journal, 46: 448-461.

Kramzar, L.M., Mueller, T., Erickson, B., Higgs, D.C. (2006) Regulatory sequences of orthologous petD mRNA chloroplast mRNAs are highly specific among Chlamydomonas species. Plant Molecular Biology, 60: 405-422.

Erickson, B., Stern, D.B., Higgs, D.C. (2005) Microarray analysis confirms the specificity of a Chlamydomonas reinhardtii chloroplast RNA stability mutant. Plant Physiol, 137: 534-544.

Hicks, A., Drager, G.D. , Higgs, D.C., Stern, D.B. (2002) An mRNA 3' processing site targets downstream sequences for rapid degradation in Chlamydomonas chloroplasts. J. Biol. Chem., 277: 3325-3333.

Swiatek, M., Kuras, R., Sokolenko, A. Higgs, D., Olive, J., Cinque, G., Müller, B., Eichacker, L.A., Stern, D.B., Bassi, R., Herrmann, R.G., and Wollman, F.-A.(2001) The chloroplast gene ycf9 encodes PSII core subunit, PsbZ, which participates in PSII supramolecular architecture. Plant Cell 13: 1347-1367.

Esposito, D., Higgs, D.C., Drager, R.B., Stern, D.B. and Jacqueline Girard-Bascou (2001) A nucleus-encoded suppressor defines a new factor that can stabilize petD mRNA in the chloroplast of Chlamydomonas reinhardtii. Current Genetics 39: 40-48.

Higgs, D.C., Shapiro, R.S., Kindle, K.L. and Stern, D.B. (1999) Small cis-acting sequences that specify secondary structures in a chloroplast mRNA are essential for RNA stability and translation. Mol. Cell. Biol., 19: 8479-8491.

Drager, R.G., Higgs, D.C., Kindle, K.L. and Stern, D.B. (1999) 5' to 3' exoribonucleolytic activity is a normal component of chloroplast mRNA decay pathways. Plant Journal, 19: 521-531.

Rott, R., Liveanu, V., Drager, R.G., Higgs, D.C., Stern, D.B. and Schuster, G. (1999) Altering the 3' UTR endonucleolytic cleavage site of a Chlamydomonas chloroplast mRNA affects 3' end maturation in vitro but not in vivo. Plant Mol. Biol., 40: 679-686.

Higgs, D.C., Kuras, R., Kindle, K.L., Wollman, F.-A. and Stern, D.B. (1998) Inversions in the Chlamydomonas chloroplast genome suppress a petD 5' untranslated region deletion by creating functional chimeric mRNAs. Plant Journal, 14: 663-671.

Higgs, D.C., Esposito, D., Kindle, K.L. and Stern D.B. (1997) RNA stability and translational regulatory elements in the 5' untranslated regions of two Chlamydomonas chloroplast transcripts. Symposium on RNA Biology II. Tool and Target. Nucleic Acid Symposium Series, 36: 132-134.

Stern, D.B., Higgs, D.C. and Yang, J. (1997) Transcription and translation in chloroplasts. Trends in Plant Science, 2: 308-315.

Higgs, D.C., Barnes, L.J. and Colbert, J.T. (1995) Abundance and half-life of the distinct oat phytochrome A3 and A4 mRNAs. Plant Mol. Biol., 29: 367-377.

Higgs, D.C. and Colbert, J.T. (1994) Oat phytochrome A mRNA degradation appears to occur via two distinct pathways. Plant Cell 6: 1007-1019.

Higgs, D.C. and Colbert, J.T. (1993) ß-glucuronidase gene expression and mRNA stability in oat protoplasts. Plant Cell Reports 12: 445-452.

Higgs, D.C. and Colbert, J.T. (1992) RNase protection assays and RNA gel blots: a direct comparison of sensitivity. Genetic Analysis: Techniques and Applications (GATA) 9: 146-148.


Higgs' Home Page

UW-Parkside Home Page

Questions, comments or suggestions should be e-mailed to David Higgs (higgs@uwp.edu)